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人小細胞肺癌細胞;NCI-H446

更新時間:2016-06-21

簡要描述:

人小細胞肺癌細胞;NCI-H446,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實驗。

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人小細胞肺癌細胞;NCI-H446
形態(tài)特性 上皮樣
生長特性 貼壁/懸浮生長,混合 
特征特性人小細胞肺癌細胞;NCI-H446該細胞是1982年由Carney D和Gazdar AF等從一位小細胞肺癌患者的胸腔積液中建立的。細胞的原始形態(tài)并不具有小細胞肺癌特征。這個細胞株是小細胞肺癌的生化和形態(tài)學(xué)上的變種,表達神經(jīng)元*的烯醇酶和腦型肌酸激酶同工酶;左旋多巴脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達到可檢測水平。與正常細胞相比,該細胞c-myc DNA序列擴增約20倍,RNA增加15倍。zui初傳代培養(yǎng)基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰島素、0.01 mg/ml轉(zhuǎn)鐵
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 1:3~1:9傳代;每周換液2~3次。
傳代情況 C5
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS 
公司細胞廣泛用于國內(nèi)院校的細胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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膠質(zhì)細胞系來源神經(jīng)營養(yǎng)因子(GDNF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17649 96T Mus musculus (Mouse)
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載脂蛋白A4(APOA4)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Apolipoprotein A4 (APOA4) X17692 96T Homo sapiens (Human)
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嘌呤能受體P2X7(P2RX7)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) X17696 96T Homo sapiens (Human)
絲聚蛋白(FLG)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Filaggrin (FLG) X17697 96T Homo sapiens (Human)
 

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